goat igg isotype r d systems polyclonal ab 108 c  (R&D Systems)

Journal: Frontiers in Immunology

Article Title: Engrafted NSG-SGM3 humanized mice spontaneously produce human immunoglobulins including IgE

doi: 10.3389/fimmu.2025.1628194

Figure Lengend Snippet: Engrafted NSG-SGM3 mice can undergo anaphylaxis with anti-human IgE alone but not by oral challenge with food allergen. (A) Change in body temperature (left panel) and clinical scores (right panel) as measures of passive systemic anaphylaxis (PSA) in engrafted NSG-SGM3 mice ("Eng") challenged with 100 ng anti-human IgE (black squares), 1 μg anti-human IgE (black triangles), or equivalent doses of isotype control (gray squares), versus non-engrafted NSG-SGM3 mice challenged with the same doses of anti-human IgE ("Non-eng"; white circles). Values represent mean ± SEM ( n = 7–14 mice/group, compiled from five independent experiments). Clinical scores range from zero to five as follows: 0) asymptomatic; 1) scratching; 2) piloerection, facial edema; 3) labored breathing, reduced activity; 4) coma or unresponsiveness; and 5) death). (B) Percentage of basophil activation for whole blood basophils from healthy human donors activated for 30 minutes with 1 µM FMLP, 1 µg/mL mouse anti-IgE, or indicated concentrations of polyclonal goat anti-human IgE ("Goat anti-IgE") or goat polyclonal IgG isotype control ("Isotype control"). Values represent mean percentage of basophils with upregulated CD63 surface expression by flow cytometry ( n = 3 human donors). (C) Change in body temperature as measure of systemic anaphylaxis for engrafted NSG-SGM3 mice challenged via oral gavage to foods to which they had positive specific IgE antibodies: cow’s milk (black squares), yeast (white circles), and hazelnut (black triangles). All clinical scores for food challenge experiments were 0. Values represent mean ± SEM ( n = 2 mice for three foods each). Error bars represent SEM.

Article Snippet: For direct targeting of IgE, engrafted and non-engrafted NSG-SGM3 mice were injected intraperitoneally with 100 ng or 1 μg of goat polyclonal anti-human IgE (Hybridoma Reagent Laboratory) or equivalent amounts of normal polyclonal goat IgG isotype control (R&D Systems) diluted in sterile 1x PBS.

Techniques: Control, Activity Assay, Activation Assay, Expressing, Flow Cytometry